Journal: Scientific Reports

Article Title: Propolis nanoemulsion and mesenchymal stem cell conditioned medium promote osteoblastogenesis against lipopolysaccharide-induced osteolysis in hyperglycemic rats

doi: 10.1038/s41598-026-46778-2

Figure Lengend Snippet: Serum concentrations of (a) ALP, (b) Coll1a1, and (c) osteopontin across treatment groups. Significant differences between groups are indicated above the bars. Abbreviations: ALP, alkaline phosphatase; Coll1a1, collagen type I alpha 1; LPS, lipopolysaccharide; NEP, nanoemulsion propolis; HUCMSCs-CM, human umbilical cord mesenchymal stem cells conditioned medium. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: This was performed following the instructions of the rat Coll1a1, ALP, and osteopontin ELISA kit (BT LAB, Shanghai Korain Biotech Co., Ltd., Shanghai, China).

Techniques:

Journal: Signal Transduction and Targeted Therapy

Article Title: Single-cell transcriptomic analysis reveals gut microbiota-immunotherapy synergy through modulating tumor microenvironment

doi: 10.1038/s41392-025-02226-7

Figure Lengend Snippet: Synergistic effects of gut microbiota and ICI treatment on SPP1. a Violin plots reveal Spp1 expression levels in myeloid subtypes across four groups. b Forest plot reveals the association between SPP1 gene expression levels and the prognosis of major cancer types in the TCGA dataset. c Kaplan–Meier survival curves show the correlation between SPP1 expression levels and prognosis in two immunotherapy cohorts. d Box plots indicate the levels of osteopontin measured by ELISA in the serum of mice across four groups. Each group consists of 4 mice. e Network diagram illustrates the differential interaction strength of the Spp1-related pathway between two cell types in the PW group compared to the others. f Bubble plot displays the interaction strength of the Spp1-related pathways between tumor-associated macrophages (TAMs)/NK cells and MC38 tumor cells in the PW group compared to the others. g Network diagram shows the differential interaction strength of the Spp1-related pathway between myeloid subtypes and MC38 cells in the PW group compared to the others. h Network diagram illustrates the differential interaction strength of the overall signaling between myeloid subtypes and MC38 cells in the PW group compared to the others

Article Snippet: The concentration of osteopontin (OPN) in mouse serum samples was quantified using the Mouse/Rat Osteopontin ELISA Kit (KE10046; Proteintech) with the following procedure.

Techniques: Expressing, Gene Expression, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures

doi: 10.3390/cells13030258

Figure Lengend Snippet: List of primary antibodies used in the present study.

Article Snippet: rabbit monoclonal anti-osteopontin , 1:1000 , Boster Biological Technology, Pleasanton, CA, USA.

Techniques:

Journal: Molecular Biomedicine

Article Title: Osteopontin promoted cardiac inflammation through increased interleukin-12 in acute myocarditis

doi: 10.1186/s43556-025-00333-z

Figure Lengend Snippet: OPN serves as a biomarker for myocarditis diagnosis. a Diagram illustrates detection of plasma OPN from acute myocarditis patients. b ELISA assay of IL-1-α, IL-1-β, IL-6 and TNF-α expression levels in plasma of acute myocarditis patients. p -value analyzed by Unpaired T-Test ( N = 6). c Echocardiographic parameters in acute myocarditis patients and Controls. LVEF: left ventricular ejection fraction. p -value analyzed by Unpaired T-Test ( N = 6). d ELISA assay of OPN levels in acute myocarditis patients. p -value analyzed by Unpaired T-Test ( N = 6). e Person analyses of correlation between OPN levels and LVEF, respectively ( N = 6). f ROC analyses determine specificity and sensitivity of OPN for acute myocarditis diagnosis ( N = 6)

Article Snippet: Plasma or cellular levels of OPN and cytokines, including IL-1β (Cat# EK0389), IL-1α (Cat# EK0392), IL-6 (Cat#EK0389), and TNF-α (Cat#EK0525), were measured using the OPN ELISA kit or corresponding cytokine ELISA kits (Boster, China) following the instruction.

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing